Primer

Part:BBa_K5427060

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


FW2 _ SS_pJump24 (I GEM_2024_8)

Forward primer for Gibson Assembly of masP1 and pJUMP24.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 55
    Illegal suffix found in sequence at 13
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 55
    Illegal SpeI site found at 14
    Illegal PstI site found at 28
    Illegal NotI site found at 21
    Illegal NotI site found at 40
    Illegal NotI site found at 61
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 55
    Illegal XhoI site found at 49
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 55
    Illegal suffix found in sequence at 14
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 55
    Illegal XbaI site found at 70
    Illegal SpeI site found at 14
    Illegal PstI site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None